Uji validasi formula pengencer spermatozoa nano- flavonoid tahan suhu ruang

Abstract

The aim of this study was to evaluate the quality of liquid semen from POGASI cattle using CEP diluent with the addition of nano-flavonoids stored at different temperatures for 3 hours. The research material used 10 POGASI cattle, housed with an artificial vagina. The requirement for fresh cement to be used is to have progressive motility >70%. There are 3 diluent formulas, namely P0: CEP diluent; P1: CEP + nano-flavonoids 0.10 grams; and P2: CEP + nano-flavonoid 0.15 grams which was tested with fresh semen. There are 2 cement storage treatments, namely at temperatures of 3-5°C and 26-28°C. Parameters measured were sperm quality (spermatozoa motility, viability, and kinetic parameters (motility, progressive motility, pH). MDA levels, DNA Fragmentase, and SEM,. The study was repeated five times, and analyzed using analysis of variance (ANOVA). The viability of spermatozoa at a temperature of 3-5°C during the third hour of storage with the average motility level with the temporary best formulation, namely treatment P1, respectively, was (68.3±6.5; 50.0±6.7; 52.2±2.2). motility level (56.6±4.7; 55.6±6.8; 46.1±7.9). Meanwhile, storage temperature of 26°C could not support spermatozoa viability until the 3rd hour 76.3±0.7), and the motility quality of the P1 treatment did not differ much in terms of motility level, namely (78.5±1.2; 78.5±1.2). Furthermore, it can be concluded temporarily that the diluent made from CEP which added nano-flavonoids, the motility at the 2nd hour was still above 45%.