Rekayasa genetika dan fermentasi mikroba: solusi bioteknologi untuk pakan ternak ramah lingkungan
DOI:
https://doi.org/10.25047/animpro.2025.866Keywords:
Biotechnology, Microbial Fermentation, Sustainable Feed, Genetic Engineering, Farming SystemsAbstract
The continuously increasing global demand for animal-based food has reinforced the urgency of developing sustainable livestock farming practices, particularly in terms of feed production. Sustainable animal diets play a crucial role in improving livestock health and productivity while reducing negative environmental impacts. Biotechnology offers various innovative approaches that hold promise for improving feed quality and efficiency, including through genetic engineering, microbial fermentation, and the utilisation of alternative feed sources. This article is a literature review aimed at exploring and analysing the integration of various biotechnological innovations into sustainable feed systems. This study discusses the potential of biotechnology in optimising the utilisation of agricultural waste, reducing greenhouse gas emissions, and decreasing reliance on synthetic inputs in feed production. Thus, this article is expected to provide scientific insights into the contribution of biotechnology in transforming livestock production systems to align with environmental and economic sustainability goals.
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Abushal, L. T., Salama, M., Essa, M. M., & Qoronfleh, M. W. (2021). Agricultural biotechnology: Revealing insights about ethical concerns. Journal of Biosciences, 46(3), 81.
Adymkhanov, L., Makarov, S., & Belousov, I. (2024). Adaptive resource-saving technologies in agriculture: The role of biotechnologies and their impact on environmental safety. BIO Web of Conferences, 140, 4006.
Afzaal, M., Iqbal, S. S., Abbasi, N. A., Sharif, F., Fatima, M., Nisar, H., Imran, A., Bashir, N., Mannan, H. A., & Khan, A. A. A. (2024). Fermentation and Production of Microorganisms for Industrial Products: An Introduction. In Recent Advances in Industrial Biochemistry (pp. 1–15). Springer.
Ahmad, S., Wei, X., Sheng, Z., Hu, P., & Tang, S. (2020). CRISPR/Cas9 for development of disease resistance in plants: recent progress, limitations and future prospects. Briefings in Functional Genomics, 19(1), 26–39.
Ahmadikhah, A., Zarabizadeh, H., Nayeri, S., & Abbasi, M. S. (2025). Advancements in genome editing tools for genetic studies and crop improvement. Frontiers in Plant Science, 15, 1370675.
Akintan, O., Gebremedhin, K. G., & Uyeh, D. D. (2024). Animal feed formulation—Connecting technologies to build a resilient and sustainable system. Animals, 14(10), 1497.
Alagawany, M., Sallam, S., & Abd El-Hack, M. E. (2024). Organic Feed Additives for Livestock. Elsevier Science & Technology.
Ashraf, U., Kiran, M., Shahid, M. N., Anjum, S. A., & Khan, I. (2023). Biofortification through seed priming in food crops: potential benefits and future scope. In Mineral Biofortification in Crop Plants for Ensuring Food Security (pp. 261–296). Springer.
Augustin, M. A., Hartley, C. J., Maloney, G., & Tyndall, S. (2024). Innovation in precision fermentation for food ingredients. Critical Reviews in Food Science and Nutrition, 64(18), 6218–6238.
Basak, A., Mondol, M. S. A., Dattta, J., Chakraborty, M., Reza, M. W., Khatun, M., De, T., & Paramanik, B. (2024). From lab to plate: harnessing plant genetic engineering for resilient and sustainable agriculture. Sustainable Agriculture: Nanotechnology, Biotechnology, Management and Food Security, 267.
Basu, S. K., Dutta, M., Goyal, A., Bhowmik, P. K., Kumar, J., Nandy, S., Scagliusi, S. M., & Prasad, R. (2010). Is genetically modified crop the answer for the next green revolution? GM Crops, 1(2), 68–79.
Bhat, R., Sharma, M., Rätsep, R., Malenica, D., & Jõgi, K. (2023). Challenges and prospects of tackling food loss and wastes in the circular economy context. Sustainable Food Value Chain Development: Perspectives from Developing and Emerging Economies, 15–36.
Bindari, Y. R., & Makkar, H. P. S. (2016). Towards implementation of sustainable animal diets: evidence generation, perspective building and way forward. CABI Reviews, 2016, 1–27.
Bogueva, D., & Danova, S. (2024). Comparing Precision Fermentation and Traditional Fermentations: Consumer Views. In Consumer Perceptions and Food (pp. 563–588). Springer.
Burggraaf, V., Lucci, G., Ledgard, S., Antille, D., Snow, V., & de Klein, C. (2021). Application of circular economy principles to New Zealand pastoral farming systems.
Byrne, P. F. (2006). Safety and public acceptance of transgenic products. Crop Science, 46(1), 113–117.
Carter, S. R., & Warner, C. M. (2018). Trends in synthetic biology applications, tools, industry, and oversight and their security implications. Health Security, 16(5), 320–333.
Ceccarelli, S. (2014). GM crops, organic agriculture and breeding for sustainability. Sustainability, 6(7), 4273–4286.
Cha, S., Lim, H. G., Kwon, S., Kim, D., Kang, C. W., & Jung, G. Y. (2021). Design of mutualistic microbial consortia for stable conversion of carbon monoxide to value-added chemicals. Metabolic Engineering, 64, 146–153.
Chandrasekaran, M. (2012). Valorization of food processing by-products. CRC press.
Chapotin, S. M., & Wolt, J. D. (2007). Genetically modified crops for the bioeconomy: meeting public and regulatory expectations. Transgenic Research, 16, 675–688.
Chaudhary, M., Mukherjee, T. K., Singh, R., Gupta, M., Goyal, S., Singhal, P., Kumar, R., Bhusal, N., & Sharma, P. (2022). CRISPR/Cas technology for improving nutritional values in the agricultural sector: an update. Molecular Biology Reports, 49(7), 7101–7110.
Chisti, Y. (2023). Biotechnology for sustainable production of food.
de Almeida, E. L. M., Ventorim, R. Z., Dias, R. G., de Moura Ferreira, M. A., Bôas, S. G. V., & da Silveira, W. B. (2024). Novel Insights About Precision Fermentation. Trending Topics on Fermented Foods, 161–200.
De Mol, M. L., Marcoen, V., Maryns, I., Snoeck, N., Beauprez, J. J., De Maeseneire, S. L., & Soetaert, W. K. (2023). Evaluation of long-term fermentation performance with engineered Saccharomyces cerevisiae strains. Fermentation, 9(8), 721.
Deshmukh, R. (2021). Impact of GM Crops on Agriculture Production: A Positive Reflection through Success Stories. In Policy Issues in Genetically Modified Crops (pp. 161–187). Elsevier.
Diez-Ozaeta, I., Berasarte, I., Zeid, A. F., Fernández, M., Russo, P., López, P., Dueñas, M. T., & Mohedano, M. L. (2025). Functional characterization of the riboflavin-overproducing and dextran-producing Weissella cibaria BAL3C-5 C120T strain for the development of biofortified plant-based beverages. International Journal of Food Microbiology, 426, 110908.
Ekici, K., & Sancak, Y. C. (2011). A perspective on genetically modified food crops. African Journal of Agricultural Research, 6(7), 1639–1642.
Fisberg, M., & Machado, R. (2015). History of yogurt and current patterns of consumption. Nutrition Reviews, 73(suppl_1), 4–7.
Frewer, L., Lassen, J., Kettlitz, B., Scholderer, J., Beekman, V., & Berdal, K. G. (2004). Societal aspects of genetically modified foods. Food and Chemical Toxicology, 42(7), 1181–1193.
Gao, Y., & Cabrera Serrenho, A. (2023). Greenhouse gas emissions from nitrogen fertilizers could be reduced by up to one-fifth of current levels by 2050 with combined interventions. Nature Food, 4(2), 170–178.
Hameed, A., Zaidi, S. S.-A., Shakir, S., & Mansoor, S. (2018). Applications of new breeding technologies for potato improvement. Frontiers in Plant Science, 9, 925.
Hansson, S. O., & Joelsson, K. (2013). Crop biotechnology for the environment? Journal of Agricultural and Environmental Ethics, 26, 759–770.
He, Z., Zhang, Y., Liu, X., de Vries, W., Ros, G. H., Oenema, O., Xu, W., Hou, Y., Wang, H., & Zhang, F. (2023). Mitigation of nitrogen losses and greenhouse gas emissions in a more circular cropping-poultry production system. Resources, Conservation and Recycling, 189, 106739.
Hocq, R., & Sauer, M. (2022). An artificial coculture fermentation system for industrial propanol production. FEMS Microbes, 3, xtac013.
Kafle, S. (2023). CRISPR/CAS9: A new paradigm for crop improvement revolutionizing agriculture. Journal of Agriculture and Food Research, 11, 100484.
Kent, L. (2004). What’s the holdup? Addressing constraints to the use of plant biotechnology in developing countries. AgBioForum, 7(1–2), 63–69.
Kerr, E. (2003). Industrial enzymes spearhead white biotech-Technology stands to revamp other industries and invigorate biotech marketplace. Genetic Engineering News, 23(16), 64-+.
Kouhen, M., García-Caparrós, P., Twyman, R. M., Abdelly, C., Mahmoudi, H., Schillberg, S., & Debez, A. (2023). Improving environmental stress resilience in crops by genome editing: insights from extremophile plants. Critical Reviews in Biotechnology, 43(4), 559–574.
KOUNDAL, K. R. (2023). Implementation of Biosafety Regulations and Guidelines of Genetically Modified Crops in India and Future Perspective. Indian J Agric Biochem, 36(2), 195–198.
Kumar, A. (2020). Synthetic biology and future production of biofuels and high–value products. In Climate Change, Photosynthesis and Advanced Biofuels: The Role of Biotechnology in the Production of Value-added Plant Bio-products (pp. 271–302). Springer.
Kumar, H., Lata, R., Khan, U., & Gond, S. K. (2024). Biotechnological approaches for crop movement and production. Sustainable Agriculture: Nanotechnology and Biotechnology for Crop Production and Protection, 335.
Li, W., Ding, K., Cao, P., & Zhao, L. (2020). Research and application progress of bacteria and enzymes synergy in feed.
Makkar, H. P. S. (2016). Smart livestock feeding strategies for harvesting triple gain–the desired outcomes in planet, people and profit dimensions: a developing country perspective. Animal Production Science, 56(3), 519–534.
Makkar, H. P. S., & Ankers, P. (2014). Towards sustainable animal diets: a survey-based study. Animal Feed Science and Technology, 198, 309–322.
Mamphogoro, T. P., Mpanza, T. D. E., & Mani, S. (2024). Animal feed production and its contribution to sustainability of livestock systems: African perspective. The Marginal Soils of Africa: Rethinking Uses, Management and Reclamation, 37–54.
Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M., & Dimitrijević-Branković, S. (2021). From agricultural waste to biofuel: enzymatic potential of a bacterial isolate Streptomyces fulvissimus CKS7 for bioethanol production. Waste and Biomass Valorization, 12, 165–174.
Minarti, A., Rinanti, A., Fachrul, M. F., Tazkiaturrizki, & Fadhila, R. (2024). Circular Economy for Biodiesel Production by Managing Wastewater Using Microalgae. In Algae as a Natural Solution for Challenges in Water-Food-Energy Nexus: Toward Carbon Neutrality (pp. 463–521). Springer.
Mishra, A., Sharma, P., Goel, C., Singh, B. P., & Arora, N. (2010). Allergenicity assessment of genetically modified foods. Int J Integr Biol, 10, 1–8.
Moretti, A. F., Brizuela, N. S., Bravo-Ferrada, B. M., Tymczyszyn, E. E., & Golowczyc, M. A. (2023). Current applications and future trends of dehydrated lactic acid bacteria for incorporation in animal feed products. Fermentation, 9(8), 742.
Moulas, A. N., & Vaiou, M. (2018). Vitamin D fortification of foods and prospective health outcomes. Journal of Biotechnology, 285, 91–101.
Najafian, L., Zamanmirabadi, A., & Khaleghi, F. (2020). An Overview of Challenges in Producing and Consuming Transgenic Products. Journal of Mazandaran University of Medical Sciences, 30(185), 154–172.
Naveen, A. K., & Sontakke, M. (2024). A review on regulatory aspects, challenges and public perception in acceptance of genetically modified foods. Food Science and Biotechnology, 33(4), 791–804.
Nerkar, G., Devarumath, S., Purankar, M., Kumar, A., Valarmathi, R., Devarumath, R., & Appunu, C. (2022). Advances in crop breeding through precision genome editing. Frontiers in Genetics, 13, 880195.
Nyende, A. B. (2008). Biotechnology in plant nutrient management for agricultural production in the tropics: The research link. African Journal of Biotechnology, 7(6).
Pandey, A., Negi, S., & Soccol, C. R. (2016). Current developments in biotechnology and bioengineering: production, isolation and purification of industrial products. Elsevier.
Paul, I., & Sarkar, P. (2024). Application of Microbial Technique for Synthesis of Food-Grade Agents from Different Agrobiomasses: An Overview. Agricultural Biomass for the Synthesis of Value-Added Materials, 300–322.
Paul, M. J., Nuccio, M. L., & Basu, S. S. (2018). Are GM crops for yield and resilience possible? Trends in Plant Science, 23(1), 10–16.
Pradhan, S., & Parida, A. (2023). Perception of Food Crops Developed by Mutagenesis Among Various Stakeholders. In TILLING and Eco-TILLING for Crop Improvement (pp. 217–236). Springer.
Qayoom, K., Baqual, M. F., Mushtaq, N., & Farooq, S. (2025). Biotechnology as a Catalyst for Sustainable Development: Leveraging Innovation for Global Goals. In Role of Science and Technology for Sustainable Future: Volume 2-Applied Sciences and Technologies (pp. 207–214). Springer.
Rakicka, M., Marko, A., Sturdik, E., Danihelova, M., Mosovska, S., & Jurikova, L. (2015). The Influence of Bacteria of Lactic Acid Fermentation on Chemical Composition of Food. CHEMICKE LISTY, 109(5), 371–376.
Ramessar, K., Capell, T., Twyman, R. M., Quemada, H., & Christou, P. (2009). Calling the tunes on transgenic crops: the case for regulatory harmony. Molecular Breeding, 23, 99–112.
Rasheed, A., Gill, R. A., Hassan, M. U., Mahmood, A., Qari, S., Zaman, Q. U., Ilyas, M., Aamer, M., Batool, M., & Li, H. (2021). A critical review: recent advancements in the use of CRISPR/Cas9 technology to enhance crops and alleviate global food crises. Current Issues in Molecular Biology, 43(3), 1950–1976.
Rizwan, D., Kirmani, S. B. R., & Masoodi, F. A. (2025). Circular Economy in the Food Systems: A Review. Environmental Quality Management, 34(4), e70096.
Robas Mora, M., Fernández Pastrana, V. M., Probanza Lobo, A., & Jiménez Gómez, P. A. (2022). Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa). Frontiers in Microbiology, 13, 1048154.
Sabat, M., & Tripathy, A. (2024). Genetically Modified and Gene-Edited Food Crops: Recent Status and Future Prospects. Food Production, Diversity, and Safety Under Climate Change, 211–222.
Sabater, C., Ruiz, L., Delgado, S., Ruas-Madiedo, P., & Margolles, A. (2020). Valorization of vegetable food waste and by-products through fermentation processes. Frontiers in Microbiology, 11, 581997.
Sairam, R. V, & Prakash, C. S. (2005). OBPC Symposium: maize 2004 & beyond—Can agricultural biotechnology contribute to global food security? In Vitro Cellular & Developmental Biology-Plant, 41, 424–430.
Saleh, N. E., & Abdel-Latif, H. M. R. (2025). Shedding light on developments in finfish nutrition. In Organic Feed Additives for Livestock (pp. 235–259). Elsevier.
Salter, A. M. (2017). Improving the sustainability of global meat and milk production. Proceedings of the Nutrition Society, 76(1), 22–27.
Schiek, B., Hareau, G., Baguma, Y., Medakker, A., Douches, D., Shotkoski, F., & Ghislain, M. (2016). Demystification of GM crop costs: releasing late blight resistant potato varieties as public goods in developing countries. International Journal of Biotechnology, 14(2), 112–131.
Sharma, M., Sidhu, A. K., & Sati, D. (2023). Prospects of biotechnology for productive and sustainable agro-environmental growth. In Advanced Microbial Techniques in Agriculture, Environment, and Health Management (pp. 83–96). Elsevier.
Sharma, P., Bano, A., Verma, K., Yadav, M., Varjani, S., Singh, S. P., & Tong, Y. W. (2023). Food waste digestate as biofertilizer and their direct applications in agriculture. Bioresource Technology Reports, 23, 101515.
Shiferaw Terefe, N., & Augustin, M. A. (2020). Fermentation for tailoring the technological and health related functionality of food products. Critical Reviews in Food Science and Nutrition, 60(17), 2887–2913.
Singh, A., Dikshit, R., & Singh, N. (2023). Valorization of Agro-Industrial Wastes for Biorefinery Products. In Green Technologies for Industrial Waste Remediation (pp. 101–123). Springer.
Smug, L. N., Salminen, S., Sanders, M. E., & Ebner, S. (2014). Yoghurt and probiotic bacteria in dietary guidelines of the member states of the European Union. Beneficial Microbes, 5(1), 61–66.
Talakayala, A., Katta, S., & Garladinne, M. (2020). Genetic engineering of crops for insect resistance: An overview. Journal of Biosciences, 45(1), 114.
Thakur, K., Wei, Z.-J., & Tomar, S. K. (2020). Riboflavin-enriched fermented soy milk for redox-mediated gut modulation: in the search of novel prebiotics. In Molecular nutrition (pp. 91–103). Elsevier.
Thapa, N., & Tamang, J. P. (2015). Functionality and therapeutic values of fermented foods. Health Benefits of Fermented Foods, 111, 168.
Tohidfar, M., & Khosravi, S. (2015). Transgenic crops with an improved resistance to biotic stresses. A review. Base.
Valerozo, J. A., Rice, D., Bucao, D. S., Ramil, R., Agrupis, S. C., & Anal, A. K. (2025). Integrative Approaches in Microbial Fermentation of Underutilized Crops for Enhanced Nutritional and Bioactive Functionalities. Food Bioscience, 106941.
Venegas, M., Leiva, A. M., Reyes-Contreras, C., Neumann, P., Piña, B., & Vidal, G. (2021). Presence and fate of micropollutants during anaerobic digestion of sewage and their implications for the circular economy: A short review. Journal of Environmental Chemical Engineering, 9(1), 104931.
Verma, R. K., Devi, R., Shukla, S., Shukla, S. K., Kumar, S., & Prajapati, A. (2017). Biotechnology application in animal nutrition and production. Biochemical & Cellular Archives, 17(1).
Wang, J., Sha, Z., Zhang, J., Qin, W., Xu, W., Goulding, K., & Liu, X. (2023). Improving nitrogen fertilizer use efficiency and minimizing losses and global warming potential by optimizing applications and using nitrogen synergists in a maize-wheat rotation. Agriculture, Ecosystems & Environment, 353, 108538.
Wenk, C. (2003). Herbs and botanicals as feed additives in monogastric animals. Asian-Australasian Journal of Animal Sciences, 16(2), 282–289.
Wiltschi, B., Cernava, T., Dennig, A., Casas, M. G., Geier, M., Gruber, S., Haberbauer, M., Heidinger, P., Acero, E. H., & Kratzer, R. (2020). Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications. Biotechnology Advances, 40, 107520.
Yadav, J., Ray, S., Soni, M., & Kashyap, B. K. (2023). Microbial fermentation system for the production of biopolymers and bioenergy from various organic wastes and by-products. In Current research trends and applications in waste management (pp. 307–339). Springer.
Zegeye, W. A., Tsegaw, M., Zhang, Y., & Cao, L. (2022). CRISPR-based genome editing: advancements and opportunities for rice improvement. International Journal of Molecular Sciences, 23(8), 4454.
Zepeda, J. F. (2006). Coexistence, genetically modified biotechnologies and biosafety: implications for developing countries. American Journal of Agricultural Economics, 88(5), 1200–1208.
Zhang, A., Xu, W., Zhao, F., & Jiang, N. (2022). Research progress of microbial fermented feed in animal production.
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Copyright (c) 2025 Rezki Amalyadi, I Gede Nano Septian, Aminurrahman Aminurrahman, Ine Karni
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