Pertumbuhan Tanaman Anggrek Cattleya (Cattleya eximia) secara In-Vitro pada Media MS dengan Subtitusi NAA dan BAP

Abstract

Orchid plants are one of good prospects commodities for the floriculture business because they have high aesthetic value. Cattleya orchid has distinctive characteristics with large, beautiful, brightly colored and fragrant flowers. In vitro propagation is one way to meet the increasing demand for orchids. In vitro culture techniques require a lot of culture media to multiply plants in large quantities, so an alternative is needed to increase the effectiveness of using media such as the addition of plant growth regulators. Therefore, research was carried out on the growth of in vitro cattleya orchids on MS media with NAA and BAP substitutions. This study aimed to determine the effect of adding NAA and BAP to MS media on the growth of in vitro cattleya orchid explants. This research was conducted from June to September 2022 at the Jember State Polytechnic Tissue Culture Laboratory. This study used completely randomized design factorial. The first factor was the NAA concentration consisted of 3 levels, namely 0, 1 and 2 ppm. The second factor was the BAP concentration consisted of 3 levels, namely 0, 2 and 4 ppm. Observational variables included plant height, the number of shoots, leaves and roots. Data were analyzed using analysis of variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at 5% level. Giving NAA 2 ppm + BAP 2 ppm was the best treatment for the number of leaves while giving NAA 2 ppm resulted in the best root growth.