Optimalisasi Metode Sterilisasi Eskplan Kelapa Sawit (Elaeis guineensis Jacq) Secara In Vitro

Abstract

Oil palm commodity (Elaeis guineensis Jacq) is one of the largest vegetable oil producing plants besides soybean seeds, sunflower seeds and coconut oil. Due to the good prospects, the farmers want to increase the productivity of oil palm plantations. However, the provision of superior and quality seeds is a problem. One good alternative to overcome this problem is the need for vegetative propagation through tissue culture techniques. This study aims to determine the effectiveness of the sterilization technique on leaf explants of the oil palm (Elaeis guineensis Jacq) in vitro which was carried out from February to March 2023 at the Tissue Culture Laboratory of the Jember State Polytechnic. The experimental design used for this study was a Non-Factorial Completely Randomized Design (CRD) consisting of 4 treatments. Each treatment consisted of 10 replications, each replication consisting of 1 bottle containing 1 explant. The treatments used were treatment A (70% alcohol; 0.1% HgCl2, 2 g/l ascorbic acid; 2% NaOCl + 0.1 g/l fungicide), B treatment (10% NaOCl; 80% alcohol; HgCl2 0.1%) treatment C HgCl2) 0.03%; NaOCl 2%; Alcohol 50%) and treatment D (70% alcohol; bayclin 10%; bayclin 20%). From the results of research conducted that treatment D (70% alcohol; bayclin 10%; bayclin 20%) showed the best results to sterilization method of oil palm explants