Analisis Kadar Prolin Pada Daun Teh Pascapanen Dalam Kondisi Hipoksia
DOI:
https://doi.org/10.25047/agropross.2025.835Kata Kunci:
Hypoxia, Tea, Tea leaves, ProlineAbstrak
GABA tea (Gamma-aminobutyric acid tea) is a type of tea specially processed to increase its GABA content, a compound known for its calming effects and potential in preventing neurological disorders, diabetes, and cancer. One method to enhance GABA levels is through hypoxic treatment, such as post-harvest leaf immersion. GABA accumulation in plants has often been linked to increased proline levels, especially under oxidative stress conditions. However, this relationship has not been widely studied in post-harvest tea leaves. This study aims to analyze proline accumulation in tea leaves subjected to low-oxygen conditions, to assess whether GABA enhancement is directly correlated with proline levels. Freshly harvested tea shoots (one bud and three leaves) were divided into four treatment groups: a control group stored at 20°C, and groups immersed in water for 8, 16, and 24 hours. Proline content was measured for each treatment. The results showed that proline levels increased immediately after harvesting but decreased with longer immersion times. These findings suggest that hypoxic conditions induced by immersion do not promote proline accumulation, in contrast to the well-documented increase in GABA under similar conditions. Therefore, proline may not serve as a direct indicator of GABA accumulation in post-harvest tea leaves.
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