Uji Mekanisme Antagonis Rizobakteri Terhadap Sclerotium rolfsii Penyebab Rebah Semai pada Tanaman Kacang Tanah

Dyah Ayu Agustin; Abdul Latief Abadi; Luqman Qurata Aini

doi:10.25047/agropross.2022.300

Uji Mekanisme Antagonis Rizobakteri Terhadap Sclerotium rolfsii Penyebab Rebah Semai pada Tanaman Kacang Tanah

Authors

Dyah Ayu Agustin , Abdul Latief Abadi , Luqman Qurata Aini

DOI:

10.25047/agropross.2022.300

Published:

19 October 2022

Issue:

Transformasi Pertanian Digital dalam Mendukung Ketahanan Pangan dan Masa Depan yang Berkelanjutan

Keywords:

antagonist, rhizobkteria, Sclerotium rolfsii

Conference Paper

Downloads

PDF

How to Cite

Agustin, D. A., Abadi, A. L., & Aini, L. Q. (2022). Uji Mekanisme Antagonis Rizobakteri Terhadap Sclerotium rolfsii Penyebab Rebah Semai pada Tanaman Kacang Tanah . Agropross : National Conference Proceedings of Agriculture, 6, 296–307. https://doi.org/10.25047/agropross.2022.300

Download Citation

Unduhan

Data unduhan belum tersedia.

Abstract

Sclerotium rolfsii is an important disease in peanut plants. S. rolfsii causes yield losses 25-50%. This research aims was to determine the mechanism of antagonists rhizobacteria in inhibiting the growth of S. rolfsii and suppressing the occurrence of damping off disease in peanut plants. The research stage include selecting rhizobacteria isolates trhough antagonistic test against S. rolfsii, observing hyphae abnormalities, analysis hydrolytic enzyme production, molecular identification 16S rRNA rhizobacteria, and test for suppression of damping off disease in peanut plants. The results showed that of the 16 rhizobacteria isolates, there were 4 isolates that were effective in inhibited the growth of S. rolfsii. Rhizobacteria that are effective in inhibited S. rolfsii cause abnormalities on hyphae in the form of hyphae colling, bending, shrinking, and lysis. Four isolates of antagonistic rhizobacteria were produce hydrolytic enzymes such as cellulase, chitinase, and protease. A total of 2 selected rhizobacteria isolates were able to increase the height plants and as many as 3 selected rhizobacteria isolates were able to increase the number of leaves of peanut plants compared to controls. Based on the disease suppression test, 4 selected rhizobacteria isolates were able to suppress the incidence of damping off disease in peanut plants equivalent to fungicides. Based on the moleculer identification 16S rRNA isolates G19, K009, R27, and R54 consecutively were identified as Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas aeruginosa, and Enterobacter asburiae.

References

Agustiansyah, A., Ilyas, S., Sudarsono, S., & Machmud, M. (2013). Karak-terisasi Rizobakteri yang Berpotensi Mengendalikan Bakteri Xanthomonas Oryzae Pv. Oryzae dan Meningkatkan Pertumbuhan Tanaman Padi. Jurnal Hama Dan Penyakit Tumbuhan Tropika, 13(1), 42–51. Retieved from https://doi.org/10.23960/j.hptt.11342-51

Agustin, D. A. (2020). Potensi Bakteri Ri-zosfer sebagai Agens Bioremediasi Herbisida Paraquat Diklorida dan Agens Antagonis Sclerotium Rolfsii Pada Tanaman Kacang Tanah. Brawijaya.

Ali, S., Hameed, S., Shahid, M., Iqbal, M., Lazarovits, G., & Imran, A. (2020). Functional Characterization of Po-tential PGPR Exhibiting Broad-Spectrum Antifungal Activity. Microbiological Research, 232, 2-19. Retieved from https://doi.org/10.1016/j.micres.2019.126389

Chanutsa, N., Phonkerd, N., & Bunyatratchata, W. (2014). Potential of Pseudomonas aeruginosa to Control Sclerotium rolfsii Causing Stem Rot and Collar Rot Disease of Tomato. Journal of Advanced Agri-cultural Technologies, 1(2), 132–135. Retieved from https://doi.org/10.12720/joaat.1.2.132-135

Dania, V. O., & Henry, E. U. (2022). Pathogenicity of Sclerotium rolfsii Isolates Causing Stem and Root Rot Disease of Cowpea (Vigna unguiculata (L.) Walp) and Management Using Trichoderma Species. Agrivita, 44(1), 105–118. Retieved from https://doi.org/10.17503/agrivita.v44i1.3372

De Curtis, F., Lima, G., Vitullo, D., & De Cicco, V. (2010). Biocontrol of Rhi-zoctonia solani and Sclerotium rolfsii on Tomato by Delivering Antagonistic Bacteria Through a Drip Irrigation System. Crop Protection, 29(7), 663–670. Retieved from https://doi.org/10.1016/j.cropro.2010.01.012

Dewata, D., Minda Azhar, & Oktavia, B. (2016). Identifikasi Molekuler Gen 16S rRNA Isolat Bakteri Pendegradasi Inulin dari Rizosfer Umbi Dahlia. Periodic, 5(2), 16–21.

Dewi, R. S., Giyanto, G., Sinaga, M. S., Dadang, D., & Nuryanto, B. (2020). Bakteri Agens Hayati Potensial ter-hadap Patogen Penting pada Padi. Jurnal Fitopatologi Indonesia, 16(1), 37–48. Retieved from https://doi.org/10.14692/jfi.16.1.37-48

Diomande and Beute, M.K., M. (1977). Comparison of Soil Plate Fungicides Screening and Field Efficacy in Control of Sclerotium rolfsii on Pea-nuts. Plant Dis. Rep., 61(5), 408–412.

Gao, X. Y., Liu, Y., Miao, L. L., Li, E. W., Hou, T. T., & Liu, Z. P. (2017). Mechanism of Anti-Vibrio Activity of Marine Probiotic Strain Bacillus pumilus H2, and Characterization of the Active Substance. AMB Express, 7(1). Retieved from https://doi.org/10.1186/s13568-017-0323-3

Gohel, V., Singh, A., Vimal, M., Ashwini, P., & Chhatpar, H. S. (2006). Bioprospecting and Antifungal Po-tential of Chitinolytic Microorgan-isms. African Journal of Biotechnology 5 (2), 54-72.

Hanudin, Budiarto, K., & Marwoto, B. (2018). Potensi Beberapa Mikroba Pemacu Pertumbuhan Tanaman se-bagai Bahan Aktif Pupuk dan Pestisida Hayati. Chemistry Journal of State University of Padang, 37(2), 59–70. Retieved from 10.21082/jp3.v37n2.2018.p59-70

Jadhav, H.P. & Sayyed, R. Z. (2016). Hydrolytic Enzymes of Rhizospheric Microbes in Crop Protection. MOJ Cell Sci Rep. 3(5), 135–136. Re-tieved from https://doi.org/10.15406/mojcsr.2016.03.00070

Kuntalini, N. R., Kalimi, K., & puspawati, N. M. (2015). Uji Antagonistik Be-berapa Rizobakteri Terhadap Sclerotium rolfsii Sacc. Penyebab Penyakit Rebah Kecambah pada Tanaman Kacang Tanah. E‐Jurnal Agroekoteknologi Tropika, 4(2), 111–123.

Le, C. N., Mendes, R., Kruijt, M., & Raaijmakers, J. M. (2012). Genetic and Phenotypic Diversity of Sclerotium rolfsii in Groundnut Fields in Central Vietnam. Plant Disease, 96(3), 389–397. Retieved from https://doi.org/10.1094/PDIS-06-11-0468

Lorito, M. (1993). Chitinolytic Enzymes Produced by Trichoderma harzianum : Antifungal Activity of Purified Endochitinase and Chitobiosidase . Phytopathology 83(3), 302-307. Retieved from https://doi.org/10.1094/phyto-83-302

Mabood, F., Zhou, X., & Smith, D. L. (2014). Microbial Signaling and Plant Growth Promotion. J. Plant Sci, 94, 1051-1063. Retieved from https://doi.org/10.4141/CJPS2013-148

Mardiah, Syamsuddin, & Efendi. (2016). Perlakuan Benih Menggunakan Ri-zobakteri Pemacu Pertumbuhan ter-hadap Pertumbuhan Vegetatif dan Hasil Tanaman Cabai Merah (Capsi-cum annuum L.). Jurnal Floratek, 11(1), 25–35.

Mehri, Z., Khodaparast, S. A., & Mousanejad, S. (2013). Genetic Di-versity in Sclerotium rolfsii Populations Based on Mycelial Compatibility Groups in Guilan Province, Iran. Iranian Journal of Plant Pathology, 49(3), 317–324. Retieved from https://doi.org/https://www.sid.ir/en/journal/ViewPaper.aspx?ID=429315

Olanrewaju, O. S., Glick, B. R., & Babalola, O. O. (2017). Mechanisms of Action of Plant Growth Promoting Bacteria. World Journal of Microbiology and Biotechnology, 33(11), 1–16. Retieved from https://doi.org/10.1007/s11274-017-2364-9

Parwati, G. A. K. C., Khalimi, K., & Adiyartasa, W. (2014). Uji Efikasi Formulasi Rizobakteri Pantoea agglomerans GTA24 dalam Mengendalikan Penyakit Rebah Semai yang Disebabkan oleh Sclerotium rolfsii pada Tanaman Kedelai. E-Jurnal Agroekoteknologi Tropika, 3(4), 218–229.

Paul, S. K., Mahmud, N. U., Gupta, D. R., Surovy, M. Z., Rahman, M., & Islam, M. T. (2021). Characterization of Sclerotium rolfsii Causing Root Rot of Sugar Beet in Bangladesh. Sugar Tech, 23(5), 1199–1205. Retieved from https://doi.org/10.1007/s12355-021-00984-6

Safni, I., & Antastia, W. (2018). In Vitro Antagonism of Five Rhizobacterial Species Against Athelia rolfsii Collar Rot Disease in Soybean. Open Agri-culture 3, 264–272.

Sahni, S., Prasad, B. D., & Ranjan, T. (2019). Biocontrol of Sclerotium rolfsii Using Antagonistic Activities of Pseudomonads. Current Journal of Applied Science and Technology, 35(5), 1–9. Retieved from https://doi.org/10.9734/cjast/2019/v35i530202

Sajeena, A., Nair, D. S., Peteti, T. S., John, J., Sudha, B., & Meera, A. V. (2021). First Report of Basal Stem Rot And Blight of Yardlong Bean (Vigna Unguiculata Subsp. Sesquipedalis) Caused by Athelia Rolfsii in India. Journal of Plant Pathology, 103(1), 337. Retieved from https://doi.org/10.1007/s42161-020-00663-7

Silaban, I. C., Aini, L. Q., & Syib’li, M. A. (2015). Pengujian Konsorsium Mikroba Antagonis untuk Mengendalikan Jamur Sclerotium rolfsii Penyebab Penyakit Rebah Semai pada Kedelai. Hpt, 3(2), 100–107.

Siregar, S. H., Mawarni, L., & Irmansyah, T. (2017). Pertumbuhan dan Produksi Kacang Tanah (Arachis hypogea L.) dengan Beberapa Sistem Olah Tanah dan Asosiasi Mikroba. Buletin Agrohorti, 5(3), 342–350. Retieved from https://jour-nal.ipb.ac.id/in-dex.php/bulagron/article/view/16472

Sultana, F., & Id, M. M. H. (2022). Assessing The Potentials of Bacterial Antagonists for Plant Growth Pro-motion , Nutrient Acquisition , and Biological Control of Southern Blight Disease in Tomato. 1–24. Re-tieved from https://doi.org/10.1371/jour-nal.pone.0267253

Sumartini. (2012). Penyakit tular tanah (Sclerotium rolfsii dan Rhizoctonia solani) pada Tanaman Kacang-Ka-cangan dan Umbi-Umbian serta Cara Pengendaliannya. Jurnal Litbang Pertanian, 31(1), 27–34.

Suwandi. (2015). Outlook Komoditas Per-tanian Subsektor Tanaman Pangan Kacang Tanah. Pusat Data dan Sis-tem Informasi Pertanian Kemen-terian Pertanian.

Tariq, M., Noman, M., Ahmed, T., & Hameed, A. (2017). Anttagonistic Features Displayed by Plant Growth Promoting Rhizobacteria (PGPR): A Review. Journal of Plant, 38–43. Re-tieved from https://doi.org/10.29328/jour-nal.jpsp.1001004

Vanegas, N. F. G., Moreno, S. M. M., Hurtado, B. E. P., Afanador, J. G. M., Aguirre, N. C., & Franco, G. M. R. (2020). Antagonism of Plant Growth Promoting Rhizobacteria Against the Causal Agent of the Vascular Wilting of Tomato Antagonism of Plant Growth Promoting Rhizobacteria Against The Causal Agent of The Vascular Wilting of Tomato. Revista Colombiana de Biotecnologia, 22(2), 35–43. Retieved from https://doi.org/10.15446/rev.colomb.biote.v22n2.79449

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Hak cipta (Copyright) artikel yang dipublikasikan di Agropross : National Conference Proceedings of Agriculture dipegang oleh penulis (Copyright by Authors) di bawah Creative Commons Attribution 4.0 International License (CC-BY). Sehingga penulis tidak memerlukan perjanjian pengalihan hak cipta yang harus diserahkan kepada redaksi.

Uji Mekanisme Antagonis Rizobakteri Terhadap Sclerotium rolfsii Penyebab Rebah Semai pada Tanaman Kacang Tanah

Authors

DOI:

Published:

Issue:

Keywords:

Conference Paper

Downloads

How to Cite

Unduhan

Abstract

References

License

Artikel paling banyak dibaca berdasarkan penulis yang sama

Official Website Of

Polije Proceeding Publications

Contact Us

Official link

Share & Follow