Kajian Konsentrasi Media dan Hormon untuk Konservasi Apel In Vitro
Authors
Hidayatul Arisah , Baiq Dina Mariana , Marry SelvawajayantiDOI:
10.25047/agropross.2021.220Published:
22 July 2021Issue:
Peningkatan Produktivitas Pertanian Era Society 5.0 Pasca PandemiKeywords:
apel, konservasi in vitro, media MSConference Paper
Downloads
Cara Mengutip
Unduhan
Abstract
Konservasi tanaman tahunan seperti apel umumnya dilakukan di lapang. Namun, karena beberapa kendala yang dipicu oleh perubahan iklim dan serangan hama dan penyakit, diperlukan sistem konservasi yang lebih efisien, misalnya secara in vitro. Konservasi in vitro adalah alternatif untuk melestarikan tanaman yang diperbanyak secara klonal seperti apel. Penelitian ditujukan untuk melihat pengaruh perbedaan konsentrasi media dan ZPT untuk konservasi apel secara in vitro. Penelitian ini dilakukan di Laboratorium Terpadu Baltjestro dari Agustus hingga Desember 2018. Media yang diuji adalah Media MS dengankonsentrasi garam penuh (FS) dan setengah (HS) dengan penambahan BA. Aksesi yang diuji adalah K60, K66, K01, K84 dan batang bawah apel. Hasil penelitian menunjukkan bahwa media MS FS lebih baik dalam mempertahankan eksplan hidup daripada HS selama empat bulan konservasi tanpa subkultur. Kombinasi MS FS dan BA 0.1 mg L-1 memberikan hasil terbaik untuk konservasi apel untuk aksesi K01 dan K66.
Referensi
Alvim, B. F. M., de Souza, A. V. V., Lima-Brito, A., Fonseca, P. T., Soares, T. L., & de Santana, J. R. F. (2020). In vitro conservation of Amburana cearensis (Fabaceae). Ciencia Rural, 50(7), 1–8. https://doi.org/10.1590/0103-8478cr20190729
Arbeloa, A., Marín, J. A., Andreu, P., García, E., & Lorente, P. (2017). In vitro conservation of fruit trees by slow growth storage. Acta Horticulturae, 1155, 101–106. https://doi.org/10.17660/ActaHortic.2017.1155.13
Benelli, C., Ozudogru, E. A., Lambardi, M., & Dradi, G. (2012). In vitro conservation of ornamental plants by slow growth storage. Acta Horticulturae, 961, 89–93. https://doi.org/10.17660/ActaHortic.2012.961.8
Cha-um, S., & Kirdmanee, C. (2007). Minimal growth in vitro culture for preservation of plant species. Fruit, Vegetable and Cereal Science and Biotechnology, 1(1), 13–25.
da Silva, T.L., Scherwinski-Pereira, J. E. (2011). In vitro conservation of Piper aduncum and Piper hispidinervum under slow‑growth conditions. Pesq. Agropec. Bras., 46(4), 384–389.
Dewi, I. S., Jawak, G. S., Purwoko, B. S., & Sabda, M. (2015). Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan. Jurnal Hortikultura Indonesia, 5(1), 21. https://doi.org/10.29244/jhi.5.1.21-28
Dobránszki, J., & Teixeira da Silva, J. A. (2010). Micropropagation of apple - A review. Biotech Adv, 28(4), 462–488. https://doi.org/10.1016/j.biotechadv.2010.02.008
Edirisinghe, E. S. C., Denagamage, C. H., & Samarasinghe, W. L. G. (2017). Effect of nutrient medium composotions in in vitro conservation of Musa spp. Annals of Sri Lanka Departement of Agriculture, 19(2), 60–70.
El-Bahr, M. K., Abd EL-Hamid, A., Matter, M. A., Shaltout, A., Bekheet, S. A., & El-Ashry, A. (2016). In vitro conservation of embryogenic cultures of date palm using osmotic mediated growth agents. Journal of Genetic Engineering and Biotechnology, 14(2), 363–370. https://doi.org/10.1016/j.jgeb.2016.08.004
El-Homosany, A. A., & Shimaa, M. K. (2016). In vitro Conservation of “ Balady ” Apple Shoot Tip Explants and Genetic Stability under Different Sugar Concentrations and Low Temperature. J.Hort. Sci. & Ornamen.Plants, 8(3), 173–178. https://doi.org/10.5829/idosi.jhsop.2016.173.178
Hao, Y. J., & Deng, X. X. (2003). Genetically stable regeneration of apple plants from slow growth. Plant Cell, Tissue and Organ Culture, 72(3), 253–260. https://doi.org/10.1023/A:1022388728497
Kļaviņa, D., & Kļaviņa, D. (2018). Ex situ conservation of endangered plant species of Latvia by slow growth storage. Environmental and Experimental Biology, 16(2), 129–138. https://doi.org/10.22364/eeb.16.12
Lopez-Puc, G. (2013). An effective in vitro slow growth protocol for conservation of the orchid epidendrum chlorocorymbos Schltr. Tropical and Subtropical Agroecosystems, 16(1), 61–68.
Mancilla-Álvarez, E., Ramírez-Mosqueda, M. A., Arano-Avalos, S., Núñez-Pastrana, R., & Bello-Bello, J. J. (2019). In vitro techniques to the conservation and plant regeneration of malanga (colocasia esculenta l. schott). HortScience, 54(3), 514–518. https://doi.org/10.21273/HORTSCI13835-18
Mengs, B. (2018). In Vitro Conservation Of Ginger (Gingiber officinale Rosc.) By Slow Growth Culture. International Journal of Innovative Pharmaceutical Sciences and Research, 6(11), 32–38. https://doi.org/DOI: 10.21276/IJIPSR.2018.06.11.728
Mona, M. H., Rania, A. T., & Ibrahim, I. A. (2016). In vitro conservation of date palm embryos under slow-growth conditions with osmotic agent and abscisic acid. International Journal of PharmTech Research, 9(10), 173–183.
Montalvo-Peniche, M. D. C., Iglesias-Andreu, L. G., Mijangos-Cortés, J. O., Nahuat-Dzib, S. L., Barahona-Pérez, F., Canto-Flick, A., & Santana-Buzzy, N. (2007). In vitro germplasm conservation of Habanero pepper (Capsicum chinense Jacq.). HortScience, 42(5), 1247–1252.
Muñoz, M., Díaz, O., Reinún, W., Winkler, A., & Quevedo, R. (2019). Slow growth in vitro culture for conservation of Chilotanum potato germplasm. Chilean J. of Agricultural Research, 79(1), 26–35. https://doi.org/10.4067/s0718-58392019000100026
Nasiruddin, M., & Islam, A. K. M. R. (2018). in Vitro Slow-Growth Conservation for Two Genotypes of Solanum Tuberosum L. Bangladesh J. Bot., 47(3), 369–380.
Negri, V., Tosti, N., & Standardi, A. (2000). Slow growth storage of single node shoots of apple genotypes . Plant Cell , Slow-growth storage of single node shoots of apple genotypes. Plant Cell, Tissue and Organ Culture, (62), 159–162.
Oseni, O. M., Pande, V., & Nailwal, T. K. (2018). A Review on Plant Tissue Culture, A Technique for Propagation and Conservation of Endangered Plant Species. International Journal of Current Microbiology and Applied Sciences, 7(07), 3778–3786. https://doi.org/10.20546/ijcmas.2018.707.438
Pan, X. J., Zhang, W. E., & Li, X. (2014). In vitro conservation of native Chinese wild grape (Vitis heyneana Roem. & Schult) by slow growth culture. Vitis - Journal of Grapevine Research, 53(4), 207–214.
Rahayu, E. S., Habibah, N. A., & Herlina, L. (2015). Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition. Biosaintifika: Journal of Biology & Biology Education, 7(1), 29–36. https://doi.org/10.15294/biosaintifika.v7i1.3541
Rezende, R. A. L. S., Rodrigues, F. A., Rezende, R. M., Soares, J. D. R., Pasqual, M., & de Assis, F. A. (2018). In vitro conservation of Cape gooseberry through slow-growth nodal segment cultures. Pesquisa Agropecuaria Brasileira, 53(5), 651–655. https://doi.org/10.1590/S0100-204X2018000500015
Rida, A. S., Qudah, T., & Al-Abdallat, A. (2015). In Vitro Conservation and Cryopreservation of Medicinal and Aromatic Plants : A Review, 11(1), 147–167. https://doi.org/10.12816/0030081
Sedlak, J., Zidova, P., & Paprstein, F. (2019). Slow growth in vitro conservation of fruit crops. Acta Hort, 1234, 119–124. https://doi.org/10.17660/ActaHortic.2019.1234.15
Lisensi
Hak Cipta (c) 2021 Hidayatul Arisah, Baiq Dina Mariana, Marry Selvawajayanti
Artikel ini berlisensi Creative Commons Attribution 4.0 International License.
Hak cipta (Copyright) artikel yang dipublikasikan di Agropross : National Conference Proceedings of Agriculture dipegang oleh penulis (Copyright by Authors) di bawah Creative Commons Attribution 4.0 International License (CC-BY). Sehingga penulis tidak memerlukan perjanjian pengalihan hak cipta yang harus diserahkan kepada redaksi.